I was making reference to the process of cyclic amplification, in a manner palatable to non biology literate individuals
The same _basic_ principles
as can be read in the chempedia example the process of RNApol replication of RNA from RNA template is error prone, thus it is superior to create a large copy number of DNA template corresponding to the mRNA desired as DNA amplification is self correcting for the most part.
dependent upon experimental or procedural requirements it may be desirable to produce error prone replication variants of the RNA template, however it is quite possible to go from RNA template to RNA product with RNApol.
due to the fragile nature of RNA there must be some shielding or , a low cycle rate is used in conjunction with immediate harvest and stabilization of the product.
the sars-2 covid19 virus replicates by way of an RNA dependent RNApol [rdRNApol]
Thanks for the additional links. I guess you would need temperature stable viral RNApol to do direct 'RNA' amplification. Not sure if that exists or if it's even possible due to RNA instability. As you say not so worthwhile due to high errors.
Producing RNA from DNA amplicons (PCR products) makes sense, I'm curious if that is the source of material from in the vaccines, or if it's artificially synthesized?
when you have determined a sequence of RNA corresponding to a desired protien you may construct a DNA template with modification to suit your purpose.
the DNA polymerase used for DNA PCR is originally from a high tempurature tolerant organism, [thermophillic bcterium] and it is possible via searching high and low to find an organism that bears high temperature tolerant rdRNApol
Thanks for the Kary Mullis story. I performed thousands of PCRs and even made some batches of taq many years ago but never knew about its ideation! I knew some pretty out there biochemists though.
to wit : https://chempedia.info/info/viral_rna_polymerase/
I was making reference to the process of cyclic amplification, in a manner palatable to non biology literate individuals
The same _basic_ principles
as can be read in the chempedia example the process of RNApol replication of RNA from RNA template is error prone, thus it is superior to create a large copy number of DNA template corresponding to the mRNA desired as DNA amplification is self correcting for the most part.
dependent upon experimental or procedural requirements it may be desirable to produce error prone replication variants of the RNA template, however it is quite possible to go from RNA template to RNA product with RNApol.
due to the fragile nature of RNA there must be some shielding or , a low cycle rate is used in conjunction with immediate harvest and stabilization of the product.
the sars-2 covid19 virus replicates by way of an RNA dependent RNApol [rdRNApol]
https://pubmed.ncbi.nlm.nih.gov/32277040/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164392/